Anti-CCND1 antibody (170-220) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.65-0.70 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	CCND1
Gene ID:	595
Uniprot ID:	CCND1_HUMAN
Immunogen Region:	170-220
Immunogen:	Synthetic peptide taken within amino acid regions 170-220 on human Cyclin D1 protein.

Post Translational Modifications	Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex. It also plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex following DNA damage. Ubiquitinated at Lys-269 by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-286 phosphorylation. The DCX(AMBRA1) complex represents the major regulator of CCND1 stability during the G1/S transition. Also ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation. Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2.leading to its stabilization.
Function	Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Protein Name	G1/S-Specific Cyclin-D1 B-Cell Lymphoma 1 Protein Bcl-1 Bcl-1 Oncogene Prad1 Oncogene
Database Links	Reactome: R-HSA-187577 Reactome: R-HSA-1912408 Reactome: R-HSA-3214858 Reactome: R-HSA-6785807 Reactome: R-HSA-69231 Reactome: R-HSA-75815 Reactome: R-HSA-8849470 Reactome: R-HSA-8853884 Reactome: R-HSA-8878166 Reactome: R-HSA-8934593 Reactome: R-HSA-8951430 Reactome: R-HSA-8951936 Reactome: R-HSA-9018519 Reactome: R-HSA-9634638 Reactome: R-HSA-9661069 Reactome: R-HSA-9754119
Cellular Localisation	Nucleus Cytoplasm Nucleus Membrane Cyclin D-Cdk4 Complexes Accumulate At The Nuclear Membrane And Are Then Translocated To The Nucleus Through Interaction With Kip/Cip Family Members
Alternative Antibody Names	Anti-G1/S-Specific Cyclin-D1 antibody Anti-B-Cell Lymphoma 1 Protein antibody Anti-Bcl-1 antibody Anti-Bcl-1 Oncogene antibody Anti-Prad1 Oncogene antibody Anti-CCND1 antibody Anti-BCL1 antibody Anti-PRAD1 antibody

Information sourced from Uniprot.org

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