Mueller-Hinton agar - Non-selective solid media for microbiology
Mueller-Hinton agar is a non-selective, non-differential microbiological growth medium. Mueller Hinton Agar contains beef extract, acid casein hydrolysate, starch and agar. Beef extract and casein acid hydrolysate provide nitrogen, vitamins, carbon, amino acids, sulphur and other essential nutrients. Starch acts as a colloid and is added to absorb any toxic metabolites produced. Hydrolysis of the starch yields dextrose, which serves as a source of energy. Agar is the solidifying agent.
Mueller-Hinton agar is commonly used for antibiotic susceptibility testing for the Kirby-Bauer disc diffusion method or standard antibiogram. Mueller-Hinton agar is therefore used for clinical diagnosis.
It is also used to isolate and maintain Neisseria and Moraxella species.
Mueller-Hinton agar can be enriched with blood (for Streptococcus), red blood cell extract (for Haemophilus).
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