General Info
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat/Goat/Sheep |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Mothers against decapentaplegic homolog 7 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | SMAD7 |
| Gene ID: | 4092 |
| Uniprot ID: | SMAD7_HUMAN |
| Specificity: | SMAD7 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from part of the human protein |
Description
| Post Translational Modifications | Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling.however it affects its ability to regulate transcription. Phosphorylated by PDPK1. Ubiquitinated by WWP1. Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1.which promotes its degradation. Acetylation prevents ubiquitination and degradation mediated by SMURF1. |
| Function | Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members.has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 7 Mad Homolog 7 Mothers Against Dpp Homolog 7 Mothers Against Decapentaplegic Homolog 8 Mad Homolog 8 Mothers Against Dpp Homolog 8 Smad Family Member 7 Smad 7 Smad7 Hsmad7 |
| Database Links | Reactome: R-HSA-201451 Reactome: R-HSA-2173788 Reactome: R-HSA-2173795 Reactome: R-HSA-2173796 Reactome: R-HSA-5689603 Reactome: R-HSA-5689880 Reactome: R-HSA-877300 |
| Cellular Localisation | Nucleus Cytoplasm Interaction With Nedd4l Or Rnf111 Induces Translocation From The Nucleus To The Cytoplasm Tgf-Beta Stimulates Its Translocation From The Nucleus To The Cytoplasm Pdpk1 Inhibits Its Translocation From The Nucleus To The Cytoplasm In Response To Tgf-Beta |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 7 antibody Anti-Mad Homolog 7 antibody Anti-Mothers Against Dpp Homolog 7 antibody Anti-Mothers Against Decapentaplegic Homolog 8 antibody Anti-Mad Homolog 8 antibody Anti-Mothers Against Dpp Homolog 8 antibody Anti-Smad Family Member 7 antibody Anti-Smad 7 antibody Anti-Smad7 antibody Anti-Hsmad7 antibody Anti-SMAD7 antibody Anti-MADH7 antibody Anti-MADH8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance