Anti-ULK1 antibody (1000-1050) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.58 µg/µl
Dilution Range:	WB: 1:250 ELISA: 1:10, 000 IP: 1:200 IF: 1:100
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	ULK1
Gene ID:	8408
Uniprot ID:	ULK1_HUMAN
Immunogen Region:	1000-1050
Immunogen:	Synthetic peptide taken within amino acid region 1000-1050 on mouse ULK1 protein.

Tissue Specificity	Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Post Translational Modifications	Autophosphorylated. Phosphorylated under nutrient-rich conditions.dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1. In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy. Ubiquitinated via 'Lys-63'-linkage by a complex composed of AMBRA1 and TRAF6 following autophagy induction, promoting ULK1 stability and kinase activity. Deubiquitinated by USP20.leading to ULK1 stability and autophagy initiation. Acetylated by KAT5/TIP60 under autophagy induction, promoting protein kinase activity.
Function	Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR.however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. Phosphorylates FLCN, promoting autophagy. Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation. Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B.
Protein Name	Serine/Threonine-Protein Kinase Ulk1 Autophagy-Related Protein 1 Homolog Atg1 Hatg1 Unc-51-Like Kinase 1
Database Links	Reactome: R-HSA-1632852 Reactome: R-HSA-5357905 Reactome: R-HSA-8854214 Reactome: R-HSA-8876198 Reactome: R-HSA-8934903
Cellular Localisation	Cytoplasm Cytosol Preautophagosomal Structure Under Starvation Conditions Is Localized To Puncate Structures Primarily Representing The Isolation Membrane That Sequesters A Portion Of The Cytoplasm Resulting In The Formation Of An Autophagosome
Alternative Antibody Names	Anti-Serine/Threonine-Protein Kinase Ulk1 antibody Anti-Autophagy-Related Protein 1 Homolog antibody Anti-Atg1 antibody Anti-Hatg1 antibody Anti-Unc-51-Like Kinase 1 antibody Anti-ULK1 antibody Anti-KIAA0722 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance