Rat CaN (Calcineurin) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	Ppp3ca
Gene ID:	24674
Uniprot ID:	PP2BA_RAT
Sample Type:	tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Tissue Specificity	Expressed in neonatal cardiomyocytes (at protein level). Expressed in hippocampal presynaptic termini (at protein level).
Post Translational Modifications
Function	Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals. Dephosphorylates heat shock protein HSPB1. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32. May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins. Required for postnatal development of the nephrogenic zone and superficial glomeruli in the kidneys, cell cycle homeostasis in the nephrogenic zone, and ultimately normal kidney function. Plays a role in intracellular AQP2 processing and localization to the apical membrane in the kidney, may thereby be required for efficient kidney filtration. Required for secretion of salivary enzymes amylase, peroxidase, lysozyme and sialic acid via formation of secretory vesicles in the submandibular glands. Required for calcineurin activity and homosynaptic depotentiation in the hippocampus. Required for normal differentiation and survival of keratinocytes and therefore required for epidermis superstructure formation. Positively regulates osteoblastic bone formation, via promotion of osteoblast differentiation. Positively regulates osteoclast differentiation, potentially via NFATC1 signaling. May play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling. Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB. Required for antigen-specific T-cell proliferation response. Dephosphorylates KLHL3, promoting the interaction between KLHL3 and WNK4 and subsequent degradation of WNK4. Negatively regulates SLC9A1 activity.
Protein Name	Protein Phosphatase 3 Catalytic Subunit Alpha Cam-Prp Catalytic Subunit Calcineurin A Alpha Calmodulin-Dependent Calcineurin A Subunit Alpha Isoform Cna Alpha Serine/Threonine-Protein Phosphatase 2b Catalytic Subunit Alpha Isoform
Database Links	Reactome: R-RNO-2025928 Reactome: -RNO-2871809 Reactome: -RNO-4086398 Reactome: -RNO-5607763
Cellular Localisation	Cytoplasm Cell Membrane Peripheral Membrane Protein Sarcolemma Myofibril Sarcomere Z Line Cell Projection Dendritic Spine Colocalizes With Actn1 And Myoz2 At The Z Line In Heart And Skeletal Muscle Recruited To The Cell Membrane By Scaffold Protein Akap5 Following L-Type Ca(2+)-Channel Activation
Alternative ELISA Names	Protein Phosphatase 3 Catalytic Subunit Alpha ELISA kit Cam-Prp Catalytic Subunit ELISA kit Calcineurin A Alpha ELISA kit Calmodulin-Dependent Calcineurin A Subunit Alpha Isoform ELISA kit Cna Alpha ELISA kit Serine/Threonine-Protein Phosphatase 2b Catalytic Subunit Alpha Isoform ELISA kit Ppp3ca ELISA kit Calna ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance